Lindsey is no stranger to fundraising as she has previously run several marathons in memory of her late husband, Leeds rugby league legend Rob Burrow.
Last year, she joined Amy to raise tens of thousands of pounds by doing a three-legged walk for Children in Need.
As the pair set off on their 80-mile (129km) trek through the region, where will the team be, and when can you see them?
Where does the challenge start?
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Lindsey and Amy will start in North Yorkshire on Monday, setting off from Ripon marketplace at 08:30 GMT.
Speaking at the start line, the pair said they were raring to go and wanted to "do Yorkshire proud".
They will walk from Ripon to Bishop Monkton, then Burton Leonard and Ripley, where they will stop for lunch.
They hope to reach Harrogate in time for a live broadcast for Look North at 18:30 before they head to Starbeck and finish in Knaresborough at about 20:00.
Where will they go next?
The team will travel through North, West and South Yorkshire this week, before finishing in Wakefield on Friday.
9 November - Ripon to Knaresborough (via Harrogate)
10 November - Sprotbrough to Conisbrough (via Mexborough)
11 November - Strines to Sheffield (via Worrall)
12 November - Meltham to Holme Moss (via Holmfirth)
13 November - Morley to Wakefield (via Ossett)
Click on the gallery above to see more locations and estimated timings for each day.
How can I donate?
You can donate any amount you like, as little as £1 can make a difference.
£30 pays for a new cot mattress for a family living in temporary accommodation, ensuring their baby has a safe place to sleep.
£50 pays for one to one play therapy for a child with a life limiting illness, helping them to cope with their condition.
£100 will pay for one to one therapy for a child who has been sexually exploited, helping them express their feelings and rebuild their life.
Get in touch
Listen to highlights from South Yorkshire on BBC Sounds, catch up with the latest episode of Look North